Proliferative Inhibition, Apoptotic Induction, Migratory Suppression of Piper nigrum Extract on HeLa Cervical Cell Line

Piper nigrum is broadly acted as a herbal medicine and anticancer activity well. Previously, we reported that extract caused induction of breast cancer cells death reduction cell migration in vitro study. In this present study, need to explore the effects on human HeLa cervical underlying mechanism. Sulforhodamine B, colony formation, cycle arrest, acridine orange/ethidium bromide staining was performed analyze cytotoxic cells. Tetraethylbenzimidazolylcarbocyanine iodide staining, dichlorodihydrofluorescein diacetate used study apoptotic followed by wound healing method, investigated migration. This crude represented effect against dose time-dependent manner with half-maximal inhibitory concentration values 48.59±5.37, 36.49±4.34, 22.71±2.12 μg/ml for 24 h, 48 h 72 respectively. Further, these viability examining decreased formation dose-dependent Next, were significantly arrested resting/growth 1 phase further attenuating mitochondrial function at incubation, especially 100 extract. Then, molecular mechanisms evaluated reactive oxygen species data showed extracts increased production amount. On other hand, suppressed observation time. These results indicated inhibited through down-regulation proliferation, apoptosis attenuation may be agent preventing/treating cancer.